π-HuB will take the snapshot of individuals’ proteomes in lifetime and investigate populations’ proteomic adaptation to major exposures on health outcomes. Specifically, π-HuB aims 1) to trace the proteome-centric trajectories in five prenatal stages including gametogenesis, fertilization, embryonic development, fetal development, and delivery, and the five postnatal stages including childhood, adolescence, pregnancy, menopause, and healthy aging; 2) to profile dynamic changes in complex proteomes during the development and progression of major diseases, including cancers, neurodegeneration, cardiovascular diseases; 3) to investigate the effects of non-genetic factors, such as the symbiotic microbiomes, lifestyles (e.g., alcohol, smoking, tea/coffee, diet, food, nutritional supplement, physical activity), and environments (e.g., hot/warm/cold climate, arid, polar/highland climate), on the human proteome as well as the underlying mechanism of short-term acclimatization and long-term adaptation.